Please click here to view a larger version of this figure. Color designations as in panel G (no plot with standard deviation option was used during the Multi Clock Scan procedure). (G) Mean clockscan profiles for all eleven ROIs selected in panel E. (F) Individual cell clock-scan profiles (composite and red, green and blue color channel profiles are shown by black, red, green and blue lines, respectively). Panels on the right show green and red channel view of the cell #7 (rectangular selection on the left panel) after the "Image | Color | Split Channels" menu function was executed. Eleven cells (see number labels) were outlined using the ImageJ polygon selection tool. 7 for the cell culture technique and Yuryev et al. (E) RGB image of cultured mouse preBI-lymphocytes, labeled with 4,6-Diamidino-2-phenylindole (DAPI, nuclear stain, blue) and with fluorescently-labeled antibodies for β1-integrin (green) and F-actin (red see Dobretsov et al. (D) Average clock-scan profile for selected ROIs (panel A) with SD bars (option "plot with standard deviation" selected). (C) Individual clock-scan profiles of five ROIs' shown in panel A. (B) A screenshot of the Multi Clock Scan window is shown when the plugin is used to analyze a grey-scale image. Red lines and numbers indicate a segmented line selections of five regions of interest in this image. For simplifying the use of the multi-clock scan plugin, these images were placed in a stack and then converted into a single image using "Image | Stacks | Make Montage" command. (A) Four fields of view were captured within the section of rat dorsal root ganglion immunostained for α3 NKA (see Figure 1A legend). Representative Example Application of using Multi Clock Scan Plugin for Image Analysis.
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